https://doi.org/10.4081/ija.2022.2011

Screening of salt tolerance traits and the salt tolerance evaluation method in Brassica napus at the seed germination stage

Vol. 17 No. 2 (2022)
Submitted: 21 November 2021
Accepted: 5 May 2022
Published: 29 June 2022
Salt tolerance, evaluation, salinity stress, germination, rapeseed (Brassica napus L).
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Authors

  • Aldiyar Bakirov State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Kazakhstan.
  • Yan Zhang State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, China.
  • Qi Zhang State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, China.
  • Shayakhmetova Altyn Seitahmetovna Agrotechnological Faculty, North Kazakhstan State University, Petropavlovsk, Kazakhstan.
  • Xiaojuan Yu 3Laboratory of Forest Microorganism, College of Forestry, Northwest A&F University, Yangling, China.
  • Yiji Shii State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, China.
  • Yu Xu State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, China.
  • Kai Wang State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, China.
  • Mengfan Qin State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, China.
  • Aixia Xu State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, China.
  • Zhen Huang • huang_zhen.8@163.com State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, China.

Soil salinity is one of the major abiotic stresses that negatively affect plant growth and agricultural productivity. For many crop species, the germination stage is one of the most sensitive stages to salinity stress. This study evaluated salt tolerance in 200 Brassica napus L. germplasms using hierarchical cluster analysis based on multiple morphological parameters, including germination rate, root length, fresh weight of root, shoot length, fresh weight of shoot, and total fresh weight. Membership function was used as a comprehensive index to select and evaluate salt tolerance of these germplasms, identifying 8 highly salt-tolerant germplasms, 40 salt-tolerant germplasms, 65 moderate salt-tolerant germplasms, 52 salt-sensitive germplasms, and 35 highly saltsensitive germplasms lines. The responses of rapeseed germplasm to salt stress indicate differences in morphological parameters. Furthermore, NaCl showed a positive effect on total fresh weight and biomass production of some germplasms at a concentration of 100 mmol L–1. Since the correlation value of salt tolerance with total fresh weight was highest under 200 mmol L–1 NaCl, it can be considered the most reliable parameter to evaluate salt tolerance. Therefore, the findings of this study can be applied as an effective and reliable method for mass screening and evaluation of Brassica napus germplasm at the germination stage for breeding salt-tolerant rapeseed genotypes.

Highlights
- The salinity tolerance of 200 varieties of B. napus germplasms was investigated.
- B. napus is more vulnerable to saline conditions during the germination and early reproductive stages than the vegetative and flowering periods.
- Based on hierarchical cluster analysis, there was a wide variability of salinity tolerance among rapeseed germplasm.
- Low concentration of sodium chloride had a positive effect on shoot and root growth, germination and total weight in some B. napus seedlings.
- Total fresh weight can be utilized as the most efficient index for mass screening of salt tolerance in B. napus germplasms at the germination stage.

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Google Scholar
Europe PMC
Ahmad I, Ibrar M, Muhammad Z, Ullah B, 2012. Effect of salinity on four cultivars of canola (Brassica napus L.) under laboratory conditions. Pak. J. Plant Sci. 18.
Arora NK, 2019. Impact of climate change on agriculture production and its sustainable solutions. Springer, Berlin, Germany. DOI: https://doi.org/10.1007/s42398-019-00078-w
Ashraf M, 1994. Organic substances responsible for salt tolerance in Eruca sativa. Biol. Plant. 36:255-9. DOI: https://doi.org/10.1007/BF02921095
Ashraf M, McNeilly T, 2004. Salinity tolerance in Brassica oilseeds. Crit. Rev. Plant Sci. 23:157-74. DOI: https://doi.org/10.1080/07352680490433286
Ashraf MY, Awan AR, Mahmood K, 2012. Rehabilitation of saline ecosystems through cultivation of salt tolerant plants. Pak. J. Bot. 44:69-75.
Bajji M, Kinet J-M, Lutts S, 2002. Osmotic and ionic effects of NaCl on germination, early seedling growth, and ion content of Atriplex halimus (Chenopodiaceae). Canad. J. Botany 80:297-304. DOI: https://doi.org/10.1139/b02-008
Bybordi A, Tabatabaei J, 2009. Effect of salinity stress on germination and seedling properties in canola cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 37:71-6.
Carpýcý E, Celýk N, Bayram G, 2009. Effects of salt stress on germination of some maize (Zea mays L.) cultivars. Afr. J. Biotechnol. 8.
Chang W, Sui X, Fan X-X, Jia T-T, Song F-Q, 2018. Arbuscular mycorrhizal symbiosis modulates antioxidant response and ion distribution in salt-stressed Elaeagnus angustifolia seedlings. Front. Microbiol. 9:652. DOI: https://doi.org/10.3389/fmicb.2018.00652
Chartzoulakis K, Loupassaki MH, 1997. Effects of NaCl salinity on germination, growth, gas exchange and yield of greenhouse eggplant. Agric. Water Manage. 32:215-25. DOI: https://doi.org/10.1016/S0378-3774(96)01276-0
Chen J, Mueller V, 2018. Coastal climate change, soil salinity and human migration in Bangladesh. Nature Climate Change 8:981-5. DOI: https://doi.org/10.1038/s41558-018-0313-8
Chen X, Min D, Yasir TA, Hu Y-G, 2012. Evaluation of 14 morphological, yield-related and physiological traits as indicators of drought tolerance in Chinese winter bread wheat revealed by analysis of the membership function value of drought tolerance (MFVD). Field Crops Res. 137:195-201. DOI: https://doi.org/10.1016/j.fcr.2012.09.008
Cuartero J, Bolarin M, Asins M, Moreno V, 2006. Increasing salt tolerance in the tomato. J. Exper. Botany 57:1045-58. DOI: https://doi.org/10.1093/jxb/erj102
Cumming R, Elliot G, 1991. Soil chemical properties. In: Charman PEV, Murphy BW (Eds.), Soils: their properties and management. Sydney University Press, Sydney, NSW, pp. 193-205:
Deng Y, Yuan F, Feng Z, Ding T, Song J, Wang B, 2014. Comparative study on seed germination characteristics of two species of Australia saltbush under salt stress. Acta Ecol. Sinica 34:337-41. DOI: https://doi.org/10.1016/j.chnaes.2013.07.011
Ding M, Hou P, Shen X, Wang M, Deng S, Sun J, Xiao F, Wang R, Zhou X, Lu C, 2010. Salt-induced expression of genes related to Na+/K+ and ROS homeostasis in leaves of salt-resistant and salt-sensitive poplar species. Plant Mol. Biol. 73:251-69. DOI: https://doi.org/10.1007/s11103-010-9612-9
Ding T, Yang Z, Wei X, Yuan F, Yin S, Wang B, 2018. Evaluation of salt-tolerant germplasm and screening of the salt-tolerance traits of sweet sorghum in the germination stage. Funct. Plant Biol. 45:1073-81. DOI: https://doi.org/10.1071/FP18009
Evelin H, Kapoor R, Giri B, 2009. Arbuscular mycorrhizal fungi in alleviation of salt stress: a review. Ann. Botany 104:1263-80. DOI: https://doi.org/10.1093/aob/mcp251
Gharoobi B, Ghorbani M, Ghasemi NM, 2012. Effects of different levels of osmotic potential on germination percentage and germination rate of barley, corn and canola. Iran. J. Plant Physiol. 2:413-7.
Guma I, Padrón-Mederos M, Santos-Guerra A, Reyes-Betancort J, 2010. Effect of temperature and salinity on germination of Salsola vermiculata L. (Chenopodiaceae) from Canary Islands. J. Arid Environ. 74:708-11. DOI: https://doi.org/10.1016/j.jaridenv.2009.10.001
Guo-Wei Z, Hai-Ling L, Lei Z, Bing-Lin C, Zhi-Guo Z, 2011. Salt tolerance evaluation of cotton (Gossypium hirsutum) at its germinating and seedling stages and selection of related indices. Yingyong Shengtai Xuebao 22.
Hamdy A, Abdel-Dayem S, Abu-Zeid M, 1993. Saline water management for optimum crop production. Agric. Water Manage. 24:189-203. DOI: https://doi.org/10.1016/0378-3774(93)90023-4
Kanwal S, Tahir MHN, Razzaq H, 2021. Principal component analysis and assessment of Brassica napus L. accessions for salt tolerance using stress tolerance indices. Pak. J. Bot. 53:113-8. DOI: https://doi.org/10.30848/PJB2021-1(10)
Khan MA, Ungar IA, 1999. Effect of salinity on seed germination of Triglochin maritima under various temperature regimes. Great Basin Natur. 144-50.
Lee J-D, Smothers SL, Dunn D, Villagarcia M, Shumway CR, Carter TE, Shannon JG, 2008. Evaluation of a simple method to screen soybean genotypes for salt tolerance. Crop Sci. 48:2194-200. DOI: https://doi.org/10.2135/cropsci2008.02.0090
Liang W, Ma X, Wan P, Liu L, 2018. Plant salt-tolerance mechanism: a review. Biochem. Biophys. Res. Commun. 495:286-91. DOI: https://doi.org/10.1016/j.bbrc.2017.11.043
Lovato MB, Martins PS, 1997. Genetic variability in salt tolerance during germination of Stylosanthes humilis HBK and association between salt tolerance and isozymes. Brazil. J. Genet. 20. DOI: https://doi.org/10.1590/S0100-84551997000300014
Maas EV, Hoffman GJ, 1977. Crop salt tolerance - current assessment. J. Irrig. Drain. Division 103:115-34. DOI: https://doi.org/10.1061/JRCEA4.0001137
Machado RMA, Serralheiro RP, 2017. Soil salinity: effect on vegetable crop growth. Management practices to prevent and mitigate soil salinization. Horticulturae 3:30. DOI: https://doi.org/10.3390/horticulturae3020030
Mahmood K, 2011. Salinity tolerance in barley (Hordeum vulgare L.): effects of varying NaCl, K+ /Na+ and NaHCO3 levels on cultivars differing in tolerance. Pak J Bot 43:1651-4.
Mahmoodzadeh H, 2008. Comparative study of tolerant and sensitive cultivars of Brassica napus in response to salt conditions. Asian J. Plant Sci. 2008:594-8. DOI: https://doi.org/10.3923/ajps.2008.594.598
Maliro MF, McNeil D, Redden B, Kollmorgen JF, Pittock C, 2008. Sampling strategies and screening of chickpea (Cicer arietinum L.) germplasm for salt tolerance. Genet. Resour. Crop Evol. 55:53-63. DOI: https://doi.org/10.1007/s10722-007-9214-9
Misra N, Dwivedi U, 2004. Genotypic difference in salinity tolerance of green gram cultivars. Plant Sci. 166:1135-42. DOI: https://doi.org/10.1016/j.plantsci.2003.11.028
Niu X, Bressan RA, Hasegawa PM, Pardo JM, 1995. Ion homeostasis in NaCl stress environments. Plant Physiol. 109:735. DOI: https://doi.org/10.1104/pp.109.3.735
Noreen Z, Ashraf M, 2007. Inter-accessional variation for salt tolerance in pea (Pisum sativum L.) at germination and screening stage. Pak. J. Botany (Pakistan). 39:2075-85.
Oku M, 2018. Branching embedding: a heuristic dimensionality reduction algorithm based on hierarchical clustering. arXiv preprint arXiv:1805.02161.
Pankova E, Konyushkova M, Gorokhova I, 2017. On the problem of soil salinity’s evaluation and method of large-scale digital mapping of saline soils. Ecosyst. Ecol. Dynamics 1.
Parida AK, Das AB, 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicol. Environ. Safety 60:324-49. DOI: https://doi.org/10.1016/j.ecoenv.2004.06.010
Pedregosa F, Varoquaux G, Gramfort A, Michel V, Thirion B, Grisel O, Blondel M, Prettenhofer P, Weiss R, Dubourg V, 2011. Scikit-learn: Machine learning in Python. J. Machine Learn. Res. 12:2825-30.
Puppala N, Fowler JL, Poindexter L, Bhardwaj HL, 1999. Evaluation of salinity tolerance of canola germination. Perspectives on new crops and new uses. ASHS Press, Alexardria, pp. 251-253.
Purty RS, Kumar G, Singla-Pareek SL, Pareek A, 2008. Towards salinity tolerance in Brassica: an overview. Physiol. Mol. Biol. Plants 14:39-49. DOI: https://doi.org/10.1007/s12298-008-0004-4
Qasim M, Ashraf M, Ashraf M, Rehman S-U, Rha E, 2003. Salt-induced changes in two canola cultivars differing in salt tolerance. Biol. Plant. 46:629-32. DOI: https://doi.org/10.1023/A:1024844402000
Raymer PL, 2002. Canola: an emerging oilseed crop. Trends N. Crops N. Uses 1:122-6.
Rebey IB, Bourgou S, Rahali FZ, Msaada K, Ksouri R, Marzouk B, 2017. Relation between salt tolerance and biochemical changes in cumin (Cuminum cyminum L.) seeds. J. Food Drug Anal. 25:391-402. DOI: https://doi.org/10.1016/j.jfda.2016.10.001
Ruiz-Lozano JM, Porcel R, Azcón C, Aroca R, 2012. Regulation by arbuscular mycorrhizae of the integrated physiological response to salinity in plants: new challenges in physiological and molecular studies. J. Exp. Botany 63:4033-44. DOI: https://doi.org/10.1093/jxb/ers126
Sabir P, Ashraf M, Akram N, 2011. Accession variation for salt tolerance in proso millet (Panicum miliaceum L.) using leaf proline content and activities of some key antioxidant enzymes. J. Agron. Crop Sci. 197:340-7. DOI: https://doi.org/10.1111/j.1439-037X.2011.00471.x
Shah AN, Tanveer M, Abbas A, Fahad S, Baloch MS, Ahmad MI, Saud S, Song Y, 2020. Targeting salt stress coping mechanisms for stress tolerance in Brassica: A research perspective. Plant Physiol. Biochem. PPB 158:53-64. DOI: https://doi.org/10.1016/j.plaphy.2020.11.044
Taarit MB, Msaada K, Hosni K, Marzouk B, 2010. Changes in fatty acid and essential oil composition of sage (Salvia officinalis L.) leaves under NaCl stress. Food Chem. 119:951-6. DOI: https://doi.org/10.1016/j.foodchem.2009.07.055
Torabi M, 2014. Physiological and biochemical responses of plants to salt stress. pp. 26-27 in Proc. of the 1st International Conference on New Ideas in Agriculture.
Wei-hua L, Hui-ming P, Jie-fu Z, Cun-kou Q, Xue-kun Z, 2013. Screening of Brassica napus for salinity tolerance at germination stage. Chinese J. Oil Crop Sci. 35:271.
Zeng L, Shannon M, Grieve C, 2002. Evaluation of salt tolerance in rice genotypes by multiple agronomic parameters. Euphytica 127:235-45. DOI: https://doi.org/10.1023/A:1020262932277
Zivdar S, Khaleghi E, Dehkordi FS, 2011. Effect of salinity and temperature on seed germination indices of Zinnia elegans L. J. Appl. Hort. 13:48-51. DOI: https://doi.org/10.37855/jah.2011.v13i01.11

How to Cite

Bakirov, A., Zhang, Y., Zhang, Q., Seitahmetovna, S. A. ., Yu, X., Shii, Y., Xu, Y., Wang, K., Qin, M., Xu, A. ., & Huang, Z. (2022). Screening of salt tolerance traits and the salt tolerance evaluation method in <em>Brassica napus</em> at the seed germination stage. Italian Journal of Agronomy, 17(2). https://doi.org/10.4081/ija.2022.2011
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