Allelopathic effects of Cynara cardunculus L. leaf aqueous extracts on seed germination of some Mediterranean weed species

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Aurelio Scavo
Alessia Restuccia
Gaetano Pandino
Andrea Onofri
Giovanni Mauromicale *
(*) Corresponding Author:
Giovanni Mauromicale | g.mauromicale@unict.it

Abstract

It is known that the presence of weeds causes serious losses to the agricultural production, both in quantitative and qualitative terms. The major problem in modern agriculture is the environmental impact of synthetic herbicides and the increase in herbicide- resistant weed species. Allelopathic compounds can be used to develop a sustainable weed management system based on natural products. The objective of this study was to evaluate the allelopathic potential of leaf aqueous extracts (40 and 80%) obtained from Cynara cardunculus L. plant species on seed germination and mean germination time of six common weeds in Mediterranean agroecosystems: Amaranthus retroflexus L., Diplotaxis erucoides (L.) DC., Portulaca oleracea L., Lavatera arborea L., Brassica campestris L. and Solanum nigrum L. Effects varied with the weed species and the concentrations of the extracts. On average, the aqueous leaf extracts significantly reduced the final percentage of seed germination compared to the control for A. retroflexus (–58.1%), D. erucoides (–43.9%) and P. oleracea (–42.5%). The rate of germination decreased with increasing extract concentration. In C. cardunculus L. var. sylvestris the autoallelopathic activity also was demonstrated. These results are very promising in order to produce a bioherbicide based on C. cardunculus allelochemicals.


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Introduction

The presence of weeds causes serious losses to the agricultural production, both in quantitative and qualitative terms, because they constantly compete spatially with crop plants, limiting the available amount of nutrients, light and moisture. Weeds are very good colonisers, reproduce faster, produce a large number of small seeds with very prolonged viability in soil and survive in the most adverse situations, becoming part of the persistent soil seedbank. On average, weeds cause a yield crop reduction estimated around 34% (Oerke, 2006). The increase in world population and the simultaneous decrease of the available resources, have led agriculture to an indiscriminate use of synthetic herbicides for weed management. This wide usage has lead to serious problems, such as the evolution of herbicide resistant weed populations and the negative impacts on environmental, human and animal health (Jabran et al., 2015). Therefore, there is an urgent need to explore ecofriendly strategies for weed control.

Putnam and Duke (1978) were the first ones to assess the possibility of using allelopathic crops for weed management in agriculture, minimising the serious problems of environmental impact. Allelochemicals includes terpenoids, N-containing compounds and phenolic compounds and can be found in different parts of plant: leaves, stems, roots, rhizomes, seeds, flowers and even pollen (Kruse et al., 2000; Bertin et al., 2003). In contrast to a high proportion of synthetic agrochemicals, allelochemicals are biodegradable, mostly water-soluble and consist of non-halogenated molecules (Bhowmik and Inderjit, 2003). Besides, they present a wide chemical diversity, are selective (Dayan et al., 2012) and, thus, may offer new mode of actions (Macias et al., 2007).

According to Rial et al. (2014), cardoon allelochemicals (primarily aguerin B, grosheimin and cynaropicrin) possess a strong phytotoxicity on the germination and growth of standard target species (tomato, lettuce, onion and watercress) and weeds (barnyardgrass and brachiaria). Moreover, the joint action of binary mixtures of aguerin B, grosheimin and cynaropicrin and one nonactive compound (11,13-dihydroxy-8-desoxygrosheimin) was also investigated on wheat coleoptide (Rial et al., 2016). Cynara cardunculus L. is a perennial diploid (2n = 2x = 34) member of Asteraceae family native to the Mediterranean Basin. According to Rottenberg and Zohary (1996), it includes the globe artichoke [var. scolymus (L.) Fiori], the cultivated cardoon (var. altilis DC.), and their progenitor wild cardoon [var. sylvestris (Lamk) Fiori]. The cultivated cardoon has been cultivated as a vegetable since ancient times, but nowadays the land area devoted to this crop is mainly localised in Spain, Italy, France and Greece (Portis et al., 2005; Mauromicale et al., 2014). Wild cardoon is a non-domesticated robust perennial plant, characterised by its rosette of large spiny leaves, branched flowering stems and blue-violet flowers.

The major product of the globe artichoke is the edible head, which is appreciated in both its fresh and processed forms (Baty-Julien and Helias, 2012). The major globe artichoke producer is Italy (about 548 Kt per year), followed by Egypt and Spain (about 391 and 200 Kt per year, respectively) (FAO, 2013). The three types are a good source of caffeoylquinic acids and flavones as reported in previous works (Schutz et al., 2004; Pandino et al., 2012; Lombardo et al., 2015). In particular, their leaves have been shown to represent a potentially productive source of polyphenols (Lombardo et al., 2009, 2015), which have various industrial, pharmaceutical and cosmetic application (Pinelli et al., 2007; Lattanzio et al., 2009). Thanks to these compounds, the C. cardunculus species have been stimulated the scientific interest at the aim to evaluate these crops as promising sources of natural antioxidant for food and not food applications. Nevertheless, the potential use of C. cardunculus leaves extracts for weed management is at the beginning of investigation.

For this reason, the purpose of this study was to evaluate the possible effects of wild cardoon, cultivated cardoon and globe artichoke allelochemicals on seed germination and mean germination time of six common weeds in Mediterranean agroecosystems. Moreover, the autoallelopathic activity on wild cardoon was considered too.

Materials and methods

Sampling of C. cardunculus plant material and preparation of aqueous leaf extracts

Fresh material was sampled from cultivated cardoon, wild cardoon and globe artichoke plants at the 25th visible leaves growth stage (November 2014), randomly, from a field crop located in the Catania University experimental station farms situated in Catania Plain [10 m (a.s.l.), 37° 25’ N, 15° 30’ E]. The three botanical varieties were at the same phenological stage. The extraction was carried out according to Sarkar et al. (2012). In the laboratory, the plant material from each botanical variety (approximately 1 kg of leaves) was washed, cut and ground. Then, a portion of each gross material was mixed with distilled water (1:10 w/v). The mixture was kept under dark conditions for 48 h at room temperature and, then, filtered through filter paper (Whatman No. 2) to eliminate the solid fraction. From this solution, two different concentrations (40 and 80%), already investigated in our preliminary studies, were obtained for each botanical variety: wild cardoon ecotype Marsala (CW 40 - CW 80), cultivated cardoon cultivar Verde de Peralta (CC 40 - CC 80) and globe artichoke cultivar Violetto di Sicilia (ART 40 - ART 80). Each extract was compared using distilled water as control (C). The prepared aqueous extracts were transferred into a falcon flask and stored in a refrigerator (3°C) for further use. In a submitted work, we identified by HPLC analysis the phytotoxic compounds of C. cardunculus, such as caffeoylquinic acids and flavones (e.g. chlorogenic acid, luteolin 7-O-glucoronide, luteoilin, apigenin 7-O-glucoronide, apigenin, etc.).

Weed seed collection

Mature seeds from adult plants were collected from six common Mediterranean weed species in natural ecosystems as well as in field crops (Table 1). Collection sites were made from natural populations in the Catania Plane of Sicily (Italy, lat. 37° 28’ N, long. 14° 57’ E, at an average altitude of 50-150 m a.s.l.). The climate of this area is of a Mediterranean type, with a long, hot and dry summer, mild winter and rain falling mostly from late autumn to early spring. Daily mean temperature during the year ranges from 8.5°C to 26°C: minimum temperature is around 0°C, while maximum can peak at over 35°C. Annual precipitation is about 500 mm (Cristaudo et al., 2007).

After collection, seeds were cleaned, kept in paper bags and dry-stored at room temperature until germination tests were performed. Seeds of each species were selected through the use of a stereomicroscope to achieve a homogeneity of the lots for size and colour.

Germination tests

Germination bioassays were arranged using 5 mL of leaf aqueous extracts at 40 and 80% and distilled water to humidify a double layer of sterilised filter paper (Whatman No. 2). Petri dishes, hermetically sealed with parafilm to prevent evaporation of the solution, were stored in incubators at the optimal conditions of temperature and photoperiod for single weed species tested. Germination tests were performed in continuous darkness and at a constant temperature of 35°C for Amaranthus retroflexus L. (Cristaudo et al., 2007) and Portulaca oleracea L. (Singh, 1973), in continuous darkness and at a constant temperature of 20°C for C. cardunculus var. sylvestris (Lekić et al., 2011), while Diplotaxis erucoides (L.) DC. (Gresta et al., 2010) and Lavatera arborea L. were incubated in alternating light (dark/light cycle 12/12 h) at 20°C and 25°C respectively. Besides, Brassica campestris L. (Kondra et al., 1983) and Solanum nigrum L. (Taab, 2009) were incubated in alternating light (dark/light cycle 8/16 h) at 20°C and 25°C respectively. Incubators maintained the designated temperature to within }1°C and they were equipped with Osram cool white fluorescent lamps with an irradiance of 25 μmol m–2 s–1, 400–750 nm.

For each treatment, four replications of 25 seeds were placed separately in 9 cm diameter plastic Petri dishes, transparent for dark/light alternating conditions and wrapped in sheets of aluminium foil for complete darkness. During the counting process, germinated seeds in continuous darkness treatments were manipulated under a green safelight (490-560 nm), while seeds in alternating photoperiod were counted during the 12-h light period (Cristaudo et al., 2016).

Germination was determined by counting and removing germinated seeds every 24 h. Germination was considered when the radicals were greater than or equal to 2 mm in length. All the determinations were performed twice and each value of a replicate is therefore a mean of the two readings.

Data analysis

The percentage of final germination (G %) was calculated as the ratio between the number of seed germinated and the total number of seeds used in each Petri dish. The corresponding proportions were analysed by way of a binomial generalised linear model with logit link (Sileshi, 2012). Plant species, allelopathic compounds and their interaction were included as factors in the model. Wherever necessary, contrasts between means were performed by using the procedures outlined in Bretz et al. (2011). The Mean Germination Times for each Petri dish were obtained by using the Kaplan-Meyer estimators (Onofri et al., 2010), together with mid-point imputation to comply with interval censoring (Law and Brookmeyer, 1992). Mean germination time (MGT) were analysed by using two-way ANOVA; a graphical inspection of residuals showed that no significant deviations with respect to the basic assumptions for ANOVA were found.

Allelopathic effect response index (RI) was calculated using the Equation (1) of Williamson and Richardson (1988):

where T is the seed germination (%) for the treated plants and C is the seed germination (%) for the corresponding control. RI ranges from –1 to +1, with positive values indicating the stimulation of germination by the aqueous extracts and negative values indicating the inhibition of germination, relative to the control.

Results and discussion

Many works report the inhibition of seed germination in presence of plant allelochemicals (Reigosa and Pazos-Malvido, 2007; Sbai et al., 2016). C. cardunculus secondary metabolites, such as chlorogenic acid and luteolin 7-O-glucoronide, have been reported to show allelopathic activity on different crops (Abdul-Rahman and Habib, 1989; Li et al., 1993; Hosni et al., 2013). In this experiment, RI was significantly affected by the interaction of species and compound (P=9.7×10–12). The RI of C. cardunculus leaf aqueous extracts was negative in all weed species under study, except in S. nigrum (and wild cardoon) (Figure 1). This variability among weed species could be attributed to the different combination of allelochemicals profile present in each extract, as well as by their level. Our hypothesis is corroborated by Ambika (2013), who found as a compound may be inhibitory at high concentration, stimulatory at low concentration, or have no effect at other concentrations.

Regardless of the weed species, all extracts reduced weed seed germination if compared with control (Figure 2). The best result was obtained with CC 80, which inhibited the weed seed germination by about 64%. On the contrary, CC 40 showed the worst allelopathic effect by reducing only 26% final seed germination, as well as the effects of ART 40, CC 40 and CW 40 appeared less marked (Figure 2). Overall, our results reported that the concentrated extract (80%) had major negative effect on weed species germination than the diluted one (40%) (45.5 vs 33.3% respectively). Similar trend was noted by Chung and Miller (1995) on selected weed species treated with alfalfa (Medicago sativa ssp. sativa L.) residues.

In A. retroflexus, all aqueous extracts significantly lowered seed germination with a decrease, on average, of 58.1% as compared to the control. ART 80 resulted the most efficient, since allowed only 17% of seed germination. Also in P. oleracea the allelopathic effect of the different solutions was significant, but not concentration-dependent. The percentage reduction of different types of extracts compared to the control was 42.5%. The lowest germination rates were obtained with CW 40 and 80 if compared with C (16 and 18% vs 61% respectively). These results are similar to many previous findings. According to Yarna et al. (2009), increasing of sorghum leaf extract concentration from 5 to 20% inhibited A. retroflexus germination from 70.76 to 92.77% and the germination time was extended too. Azizi and Fuji (2006) found that germination of A. retroflexus and P. oleracea was completely inhibited at a concentration of 0.7% (v/v) and higher of Eucalyptus globulus Labill. essential oils. Besides, they found that the undiluted hydro-alcoholic extract of Hypericum perforatum L. and Salvia officinalis L. had a significant inhibitory effect on seed germination percentage for A. retroflexus, but not for P. oleracea. Dadkhah and Asaadi (2010) reported that foliar aqueous extract of Eucalyptus camaldulensis Dehnh. not affected the germination percentage of P. oleracea, but severely reduced, especially at the higher aqueous extract concentration, the growth of young seedlings. Therefore, differently from other plant species, the C. cardunculus botanical varieties have a strong inhibitory effect on P. oleracea.

No studies about the allopathic effects on seed germination of D. erucoides have been published. Nevertheless, several works have been conducted upon the allelopathic activity of D. erucoides on field crops (Giordano et al., 2005; Qasem, 2007). In this experiment, germination of D. erucoides was significantly reduced by all aqueous extracts (66.3% control vs 29.1% average of aqueous extracts). The best results were obtained with ART 80 and CC 40 if compared with C (16.3% and 18.8% vs 66.3% respectively). However, the less marked effects were registered with CW 80. Results provide evidence of globe artichoke’s foliar extracts strong allelopathic effect on D. erucoides seed germination. That is important under the applicative aspect because D. erucoides is one of the most harmful weed in Mediterranean environments.

Allelopathic effects of C. cardunculus extracts on L. arborea seed germination and mean germination time were not significant. Therefore, L. arborea cannot be considered a target plant for C. cardunculus foliar extracts. Also in S. nigrum and B. campestris, as well as in L. arborea, the germination percentage was not significantly affected by any extracts. The low seed germination percentages of S. nigrum and B. campestris, are probably due to the high seed dormancy of wild ecotypes. These results are in contrast with Gonzalez et al. (1997) and Gao et al. (2009). The first found that the effects of six phenolics compounds obtained from the soil solution of nine pepper (Capsicum annuum L.) varieties on germination of S. nigrum were inhibitory only at a concentration of 10–2 M. The second reported that B. campestris seed germination and seed germination speed are strongly inhibited by Hemistepta lyrata Bunge water extract. B. campestris seed germination was inhibited (50%) also by aqueous extract of leaves of Parthenium hysterophorus L. at 2% concentration (Maharjan et al., 2007). Therefore, S. nigrum and B. campestris, are very sensitive to allelochemicals of some plant species, but not to C. cardunculus botanical varieties.

In addition to the reduction of germination, the delay in seed germination is crucial in weed control and can affect the ability of the seedlings to establish themselves in natural conditions (Escurdero et al., 2000; Chaves et al., 2001). Table 3 shows how C. cardunculus extracts increased MGT of A. retroflexus, P. oleracea e D. erucoides. Since S. nigrum and B. campestris seeds showed low germination percentage values, their MGTs were not considered. In A. retroflexus, all aqueous extracts increased MGT (from 1.8 d of control to 5.9 d on average of the aqueous extracts) and the best results were obtained with CC 40, CC 80 and ART 80 (7.5 d, 7.0 d and 7.0 d respectively). Also in P. oleracea, TMG was increased by all treatments (from 3.7 d of control to 5.4 d on average of treatments) and CW 40 showed the highest value (8.6 d). The most significant effect of C. cardunculus extracts on D. erucoides MGT was reached by using ART 80 and CW 80 (7.3 d and 7.2 d respectively).

In this experiment, the autoallelopathic effect on wild cardoon was also evaluated (Table 4). Our results showed that all the other leaf extracts, excluding ART 40, decreased the germination percentage if compared with C (19.6% vs 28% respectively). The most significant result (9%) was obtained with CC 80, followed by CW 40 (16%). Therefore, C. cardunculus L. shows an autoallelopathic capacity, although it is low. Autoallelopathy is probably a mechanism that helps C. cardunculus invasion in natural ecosystem through maintaining seed dormancy when conditions are not conducive to growth or by increasing plant resistance to pathogens (Friedman and Waller, 1985).

Conclusions

The present study exploited the allelopathic effect of leaf aqueous extracts of C. cardunculus on six common Mediterranean weeds. Overall, the inhibitory effect was concentration-dependent, even if different behaviour was observed among the considered weed seeds. These results are very promising in order to produce a bioherbicide based on C. cardunculus extracts. It will make possible to reduce the use of synthetic herbicides and, as consequence, should have an impact positively on the environment and human health. Nevertheless, the trial was carried out in vitro conditions, thus, further investigations are needed to determine the effect of C. cardunculus allelochemicals in open field, in order to set up the best dose-response effect on common Mediterranean weeds. Future developments involve also an accurate study on the methodology for extraction of C. cardunculus allelochemicals, particularly on the utilisation of different solvents, on the stability of the extracts, as well as analysing the polyphenol profiles and investigating the phytotoxic effects of several genotype extracts from the three botanical varieties at different growth stages and stress conditions.

References

Figure 1.: The influence of leaf aqueous extracts of C. cardunculus on the allelopathic effect response index (RI) in six weed species. The pooled standard error of the above means was 0.111. ART 40, globe artichoke extract 40%; CW 40, wild cardoon extract 40%; CC 40, cultivated cardoon 40%; ART 80, globe artichoke extract 80%; CW 80, wild cardoon extract 80%; CC 80, cultivated cardoon 80%.
Figure 2.: Percentage of reduction respect to control of weed seed germination in relation to extract of C. cardunculus. ART 40, globe artichoke extract 40%; CW 40, wild cardoon extract 40%; CC 40, cultivated cardoon 40%; ART 80, globe artichoke extract 80%; CW 80, wild cardoon extract 80%; CC 80, cultivated cardoon 80%.
Table 1.: List of the plants used in the germination tests.
Common name Scientific name Family Biological form Corotype
Redroot pigwed Amaranthus retroflexus L. Amaranthaceae T scap. Cosmop.
Purslane Portulaca oleracea L. Portulacaceae T scap. Subcosmop.
White wall rocket Diplotaxis erucoides (L.) DC. Brassicaceae T scap. W-Medit. (Steno)
Tree mallow Lavatera arborea L. Malvaceae H bien. Steno-Medit.
Field mustard Brassica campestris L. Brassicaceae T scap./H scap. Medit.
Black nightshade Solanum nigrum L. Solanaceae T scap. Cosmop. (synanthrop.)
Wild cardoon C. cardunculus L. var. sylvestris Asteraceae H scap. Steno-Medit.
Table 2.: Effects of leaf aqueous extract of C. cardunculus on seed germination (G%) of six weed species.
Leaf aqueous extract Amaranthus retroflexus Portulaca oleracea Diplotaxis erucoides Lavatera arborea Solanum nigrum Brassica campestris
Control 94.0±2.37d 61.0±4.88d 66.3±5.29d 47.0±4.99a 4.0±2.26a 8.8±3.16a
ART 40 73.0±4.44c 26.0±4.39ab 35.0±5.33c 47.0±4.99a 1.3±1.32a 3.8±2.12a
CW 40 78.0±4.14c 16.0±3.67a 37.5±5.41c 43.0±4.95a 2.7±1.86a 3.8±2.12a
CC 40 83.0±3.76c 30.0±4.58bc 18.8±4.36ab 41.0±4.92a 6.7±2.88a 2.5±1.75a
ART 80 17.0±3.76a 31.0±4.62bc 16.3±4.12a 47.0±4.99a 6.7±2.88a 5.0±2.44a
CW 80 37.0±4.83b 18.0±3.84a 40.0±5.48c 43.0±4.95a 4.0±2.26a 0.00
CC 80 40.0±4.90b 35.0±4.77c 30.0±5.12bc 35.0±4.77a 0.00 0.00

ART 40, globe artichoke extract 40%; CW 40, wild cardoon extract 40%; CC 40, cultivated cardoon 40%; ART 80, globe artichoke extract 80%; CW 80, wild cardoon extract 80%; CC 80, cultivated cardoon 80%. Values are given as means±standard error. a-dDifferent letters indicate statistical significance for P≤0.05.

Table 3.: Effects of aqueous extract of C. cardunculus on mean germination time (MGT days) of four weed species.
Leaf aqueous extract Amaranthus retroflexus Portulaca oleracea Diplotaxis erucoides Lavatera arborea
Control 1.8a 3.7a 5.4ab 6.9a
ART 40 5.0bc 4.2a 2.3a 7.3a
CW 40 4.2ab 8.6b 4.5ab 7.8a
CC 40 7.5c 4.3a 5.4ab 6.5a
ART 80 7.0bc 4.4a 7.3d 6.3a
CW 80 5.0abc 6.3ab 7.2b 8.6a
CC 80 7.0bc 4.4a 6.1b 8.2a

ART 40, globe artichoke extract 40%; CW 40, wild cardoon extract 40%; CC 40, cultivated cardoon 40%; ART 80, globe artichoke extract 80%; CW 80, wild cardoon extract 80%; CC 80, cultivated cardoon 80%. Values are given as means with pooled standard error of a mean (SEM) =1.02. a-cDifferent letters indicate statistical significance for P≤0.05.

Table 4.: Autoallelopathic effect of leaf aqueous extract of C. cardunculus on response index (RI), on seed germination (G%) and mean germination time (MGT days).
Leaf aqueous extract Wild cardoon
RI G % MGT d
Control 28.0±4.49c 9.8±1.02ab
ART 40 0.07 30.0±4.58c 9.9±1.02b
CW 40 –0.43 16.0±3.67ab 9.5±1.02ab
CC 40 –0.29 20.0±4.00bc 7.9±1.02ab
ART 80 –0.25 21.0±4.07bc 6.9±1.02a
CW 80 –0.21 22.0±4.14bc 9.3±1.02ab
CC 80 –0.68 9.0±2.86a 9.5±1.02ab

ART 40, globe artichoke extract 40%; CW 40, wild cardoon extract 40%; CC 40, cultivated cardoon 40%; ART 80, globe artichoke extract 80%; CW 80, wild cardoon extract 80%; CC 80, cultivated cardoon 80%. Values are given as means±standard error. a-cDifferent letters indicate statistical significance for P≤0.05.