Morpho-physiological and molecular responses of two Libyan bread wheat cultivars to plant growth regulators under salt stress

  • El Hadi Hadia National Institute of Agronomic Research of Tunisia, University of Carthage, Ariana, Tunisia; Faculty of Science, University of Carthage, Bizerte, Tunisia.
  • Amor Slama | slamaamor@yahoo.fr Faculty of Science, University of Carthage, Bizerte, Tunisia. https://orcid.org/0000-0002-9386-5964
  • Leila Romdhane National Institute of Agronomic Research of Tunisia, University of Carthage, Ariana, Tunisia. https://orcid.org/0000-0001-5147-5688
  • Hatem Cheikh M’hamed National Institute of Agronomic Research of Tunisia, University of Carthage, Ariana, Tunisia.
  • Ahmed Houssein Abodoma Department of Genetics, Faculty of Agriculture, University of In Shams, Egypt.
  • Mohamed Ali Saed Fahej Biology Department, College of Science, University of Elmergib, Khoms, Libya.
  • Leila Radhouane National Institute of Agronomic Research of Tunisia, University of Carthage, Ariana, Tunisia. https://orcid.org/0000-0001-8911-516X

Abstract

To study the effects of salt stress and plant growth regulators (kinetin, gibberellic acid, potassium) on growth, yield, glycine betaine content, phosphoenolpyruvate carboxylase (PEPC) and ribulose biphosphate carboxylase (RBC) gene expression of two Libyan bread wheat varieties, a factorial design of greenhouse experiment with three replications was conducted. Results revealed that salt stress significantly reduced plant growth and productivity of both varieties. Moreover, the addition of kinetin + potassium and gibberellic acid + potassium had improved the performance of the morpho-metric parameters of both genotypes under salt stress; but the performance was more effective for kinetin treatment than for gibberellic acid. At the biochemical level, the results showed that salt stress increased glycine betaine contents in both varieties with different proportions. This increase is more elevated in the presence of kinetin + potassium than the treatment with gibberellic acid+ potassium, which showed an almost similar result as in only salt stress. At the molecular level, the effects of salt stress and plant growth regulators on the PEPC and RBC gene expression showed that the increase was significantly higher for kinetin, gibberellic acid, and salt stress when compared to the control.


Highlights

- Salt stress reduced plant growth and productivity of bread wheat varieties.
- Growth regulator improved the performance of the morphometric parameters.
- The performance was more effective for kinetin treatment than for gibberellic acid.
- Kinetin improved the glycine betaine gene expression more than gibberellic acid.
- Kinetin increased significantly the phosphoenolpyruvate carboxylase and ribulose biphosphate carboxylase gene expression.

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Published
2020-09-15
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Original Articles
Keywords:
Libyan bread wheat, glycine betaine, ribulose biphosphate carboxylase, phosphoenolpyruvate carboxylase, plant growth regulator, salinity.
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How to Cite
Hadia, E. H., Slama, A., Romdhane, L., M’hamed, H. C., Abodoma, A. H., Fahej, M. A. S., & Radhouane, L. (2020). Morpho-physiological and molecular responses of two Libyan bread wheat cultivars to plant growth regulators under salt stress. Italian Journal of Agronomy, 15(3), 246-252. https://doi.org/10.4081/ija.2020.1633