Phosphorus deficiency enhances water deficit impact on some morphological and physiological traits in four faba bean (Vicia faba L.) varieties

Abstract

Highlights
- Varieties tolerant to low phosphorus and water deficiency are needed in arid and semi-arid regions with low P.
- Phosphorus nutrition is advantageous for mitigating the effect of water deficit on faba bean (Vicia faba) plants and conversely, deficient P supply reduces resilience to water deficit.
- The faba bean variety Aguadulce showed high tolerance to the combined effect of water deficit and phosphorus deficiency in terms of growth, leaf water potential, stomatal conductance, membrane permeability and glycine betaine accumulation.
- Reina Mora was the least tolerant variety to water deficit combined with P limitation.
- Combined limitation of water and phosphorus induced accumulation of glycine betaine in leaves.

 

Moroccan soils, generally present low available phosphorus (P) levels which occur in almost all arid and semi-arid regions. Faba bean is one of the most significant crops in Morocco and is influenced by these constraints that affect its nutrient uptake and nitrogen fixation capacity and hence plants development. Therefore, we evaluated the response of four Vicia faba varieties - Aguadulce (Ag), Alfia (Al), Luz de Otono (LO) and Reina Mora (RM) -, grown under biological nitrogen fixation to water deficit and two phosphorus levels. The trial was conducted under greenhouse conditions and water stress was induced by keeping pots at 40% substrate field capacity (FC) versus 80% FC for the controls while phosphorus deficiency treatment consisted in the application of 25 μmol P plant−1 week−1versus 125 μmol P plant−1 week−1 for sufficient P treatment. The results revealed a significant effect of water deficit and phosphorus deficiency either alone or combined on plants dry weights, leaf water parameters and nutrient concentrations. However sufficient phosphorus supply mitigated the adverse effects of water deficit on faba bean. We noticed significant differences between the studied varieties. Ag showed high performance concerning dry weights (1.25 g and 1.88 g plant–1 respectively for shoot and root) and high concentration of nitrogen N (4.7%) and P (0.27 mg g–1 DW) and was then qualified as the most tolerant variety to water deficit combined with P limitation. While RM was the least tolerant variety, as it showed the lowest dry weights (0.51 g and 1.4 g plant–1 respectively for shoot and root) and concentration (2.74% and 0.19 mg g–1 DW respectively for N and P). The tolerance was related to the ability to ensure efficient osmoregulation by glycine betaine accumulation, to keep leaf water balance and cell membrane stability that contribute together with adequate symbiotic nitrogen fixation to plant growth performance under combined stresses.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

References

Alghamdi S, Al-Shameri M, Migdadi H, Ammar M, El-Harty E, Khan M, Farooq M, 2015. Physiological and molecular characterization of faba bean (Vicia faba L.) genotypes for adaptation to drought stress. J. Agron. Crop Sci. 201:401-9. DOI: https://doi.org/10.1111/jac.12110

Al-Niemi TS, Kahn ML, McDermott TR, 1998. Phosphorus uptake by bean nodules. Plant Soil. 198:71-8. DOI: https://doi.org/10.1023/A:1004200903458

Antolín MC, Muro I, Sánchez-Díaz M, 2010. Application of sewage sludge improves growth, photosynthesis and antioxidant activities of nodulated alfalfa plants under drought conditions. Environ. Exp. Bot. 68:75-82. DOI: https://doi.org/10.1016/j.envexpbot.2009.11.001

Argaw A, Mnalku A, 2017. Effectiveness of native Rhizobium on nodulation and yield of faba bean (Vicia faba L.) in Eastern Ethiopia. Arch. Agron. Soil Sci. 63:1390-403. DOI: https://doi.org/10.1080/03650340.2017.1287353

Bargaz A, Drevon JJ, Oufdou K, Mandri B, Faghire M, Ghoulam C, 2011. Nodule phosphorus requirement and O2 uptake in common bean genotypes under phosphorus deficiency. Acta Agr. Scand. B-S. P. 61:602-11. DOI: https://doi.org/10.1080/09064710.2010.533188

Bargaz A, Zaman-Allah M, Farissi M, Lazali M, Drevon JJ, Maougal RT, Georg C, 2015. Physiological and molecular aspects of tolerance to environmental constraints in grain and forage legumes. Int. J. Mol. Sci. 16:18976-9008. DOI: https://doi.org/10.3390/ijms160818976

Blum A, 2017. Osmotic adjustment is a prime drought stress adaptive engine in support of plant production. Plant. Cell. Environ. 40:4-10. DOI: https://doi.org/10.1111/pce.12800

Bohnert HJ, Jensen RG, 1996. Strategies for engineering water-stress tolerance in plants. Trends Biotechnol. 14:89-97. DOI: https://doi.org/10.1016/0167-7799(96)80929-2

Chen THH, Murata N, 2011. Glycinebetaine protects plants against abiotic stress: mechanisms and biotechnological applications. Plant. Cell. Environ. 34:1-20. DOI: https://doi.org/10.1111/j.1365-3040.2010.02232.x

Farissi M, Bouizgaren A, Faghire M, Bargaz A, Ghoulam C, 2013. Agrophysiological and biochemical properties associated with adaptation of Medicago sativa populations to water deficit. Turk. J. Bot. 37:1166-75. DOI: https://doi.org/10.3906/bot-1211-16

Food and Agriculture Organization of the United Nations, 2017. FAOSTAT Database. Available from: www.fao.org/faostat/ Accessed: 21 January 2018.

Fujita K, Okada M, Lei K, Ito J, Ohkura K, Adu‐Gyamfi JJ, Mohapatra PK, 2003. Effect of P deficiency on photoassimilate partitioning and rhythmic changes in fruit and stem diameter of tomato (Lycopersicon esculentum) during fruit growth. J. Exp. Bot. 54:2519-28. DOI: https://doi.org/10.1093/jxb/erg273

Ghoulam C, Foursy A, Fares K, 2002. Effects of salt stress on growth, inorganic ions and proline accumulation in relation to osmotic adjustment in five sugar beet cultivars. Environ. Exp. Bot. 47:39-50. DOI: https://doi.org/10.1016/S0098-8472(01)00109-5

Giri J, 2011. Glycinebetaine and abiotic stress tolerance in plants. Plant Signal. Behav. 6:1746-1751. DOI: https://doi.org/10.4161/psb.6.11.17801

Grieve CM, Grattan SR, 1983. Rapid assay for determination of water soluble quaternary ammonium compounds. Plant Soil. 70:303-7. DOI: https://doi.org/10.1007/BF02374789

Gu M, Rom CR, Robbins JA, 2003. Leaf gas exchange and stomatal characteristics of six birch taxa under difference irrigation regimes. Ark. Agr. Exp. Sta. Res. Series. 506:14-16.

Gunawardena SFBN, Danso SKA, Zapata F, 1992. Phosphorus requirement and nitrogen accumulation by three mung bean (Vigna radiata L.) cultivars. Plant Soil. 147:267-74. DOI: https://doi.org/10.1007/BF00029078

Herridge DF, Peoples MB, Boddey RM, 2008. Global inputs of biological nitrogen fixation in agricultural systems. Plant Soil. 311:1-18. DOI: https://doi.org/10.1007/s11104-008-9668-3

Hinsinger P, 2001. Bioavailability of soil inorganic P in the rhizosphere as affected by root-induced chemical changes: a review. Plant Soil. 237:173-95. DOI: https://doi.org/10.1023/A:1013351617532

Israel DW, 1993. Symbiotic dinitrogen fixation and host-plant growth during development of and recovery from phosphorus deficiency. Physiol. Plantarum. 88:294-300. DOI: https://doi.org/10.1034/j.1399-3054.1993.880213.x

Jaleel CA, Manivannan P, Wahid A, Farooq M, Somasundaram R, Panneerselvam R, 2009. Drought stress in plants: a review on morphological characteristics and pigments composition. Int. J. Agric. Biol. 11:100-5.

Jemo M, Sulieman S, Bekkaoui F, Olomide OAK, Hashem A, Abd_Allah EF, Alqarawi AA, Tran L-SP, 2017. Comparative analysis of the combined effects of different water and phosphate levels on growth and biological nitrogen fixation of nine cowpea varieties. Front. Plant. Sci. 8:2111. DOI: https://doi.org/10.3389/fpls.2017.02111

Jensen ES, Peoples MB, Boddey RM, Gresshoff PM, Hauggaard-Nielsen H, Alves BJR, Morrison MJ, 2012. Legumes for mitigation of climate change and the provision of feedstock for biofuels and biorefineries. A review. Agron. Sustain. Dev. 32:329-64. DOI: https://doi.org/10.1007/s13593-011-0056-7

Jiang Y, Huang B, 2001. Drought and heat stress injury to two cool-season turfgrasses in relation to antioxidant metabolism and lipid peroxidation. Crop Sci. 41:436-42. DOI: https://doi.org/10.2135/cropsci2001.412436x

Jifon JL, Syvertsen JP, 2003. Moderate shade can increase net gas exchange and reduce photoinhibition in citrus leaves. Tree Physiol. 23:119-27. DOI: https://doi.org/10.1093/treephys/23.2.119

Kabbadj A, Makoudi B, Mouradi M, Pauly N, Frendo P, Ghoulam C, 2017. Physiological and biochemical responses involved in water deficit tolerance of nitrogen-fixing Vicia faba. PLoS One. 12:e0190284. DOI: https://doi.org/10.1371/journal.pone.0190284

Kido ÉA, Ferreira-Neto JRC, da Silva MD, Santos VEP, da Silva Filho JLB, Benko-Iseppon AM, 2019. Osmoprotectant-related genes in plants under abiotic stress: expression dynamics, In silico genome mapping and biotechnology. In: M. Hossain, V. Kumar, D. Burritt, M. Fujita, P. Mäkelä (Eds.), Osmoprotectant-mediated abiotic stress tolerance in plants. Springer, Cham. 342:1-40. DOI: https://doi.org/10.1007/978-3-030-27423-8_1

Kirkby EA, Johnston AE, 2008. Soil and fertilizer phosphorus in relation to crop nutrition. In: White P.J., Hammond J.P. (Eds.), The ecophysiology of plant-phosphorus interactions. Plant Ecophysiology, Vol. 7. Springer, Dordrecht, pp 177-223. DOI: https://doi.org/10.1007/978-1-4020-8435-5_9

Kirnak H, Cengiz K, David H, Sinan G, 2001. A long-term experiment to study the role of mulches in physiology and macronutrition of strawberry grown under water stress. Aust. J. Agricult. Res. 52:937-43. DOI: https://doi.org/10.1071/AR01014

Koivunen E, Partanen K, Perttilä S, Palander S, Tuunainen P, Valaja J, 2016. Digestibility and energy value of pea (Pisum sativum L.), faba bean (Vicia faba L.) and blue lupin (narrow-leaf) (Lupinus angustifolius) seeds in broilers. Anim. Feed Sci. Technol. 218:120-7. DOI: https://doi.org/10.1016/j.anifeedsci.2016.05.007

Kubure TE, Raghavaiah CV, Hamza I, 2016. Production potential of faba bean (Vicia faba L.) varieties in relation to plant densities and phosphorus nutrition on vertisols of Central Highlands of West Showa Zone, Ethiopia, East Africa. Adv. Crop Sci. Tech. 4:214.

Li RH, Guo PG, Baumz M, Grando S, Ceccarelli S, 2006. Evaluation of chlorophyll content and fluorescence parameters as indicators of drought tolerance in barley. Agr. Sci. China. 5:751-7. DOI: https://doi.org/10.1016/S1671-2927(06)60120-X

Makoudi B, Kabbadj A, Mouradi M, Amenc L, Domergue O, Blair M, Drevon JJ, Ghoulam C, 2018. Phosphorus deficiency increases nodule phytase activity of faba bean-rhizobia symbiosis. Acta Physiol. Plant. 40:63. DOI: https://doi.org/10.1007/s11738-018-2619-6

Mouradi M, Farissi M, Bouizgaren A, Makoudi B, Kabbadj A, Very AA, Sentenac H, Qaddoury A, Ghoulam C, 2016. Effects of water deficit on growth, nodulation and physiological and biochemical processes in Medicago sativa-rhizobia symbiotic association. Arid Land Res. Manag. 30:193-208. DOI: https://doi.org/10.1080/15324982.2015.1073194

Mouradi M, Farissi M, Khadraji A, Makoudi B, Ghoulam C, 2018. Biochemical and antioxidant proprieties associated with the adaptation of faba bean (Vicia faba L.) - rhizobia symbiosis to phosphorus deficit. J. Mater. Environ. Sci. 9:1574-81.

Multari S, Stewart D, Russell WR, 2015. Potential of Fava bean as future protein supply to partially replace meat intake in the human diet. Compr. Rev. Food Sci. Food Saf. 14:511-22. DOI: https://doi.org/10.1111/1541-4337.12146

Murata N, Takahashi S, Nishiyama Y, Allakhverdiev SI, 2007. Photoinhibition of photosystem II under environmental stress. B.B.A.-Bioenergetics. 1767:414-21. DOI: https://doi.org/10.1016/j.bbabio.2006.11.019

Nasr Esfahani M, Sulieman S, Schulze J, Yamaguchi-Shinozaki K, Shinozaki K, Tran LS, 2014. Mechanisms of physiological adjustment of N2 fixation in Cicer arietinum L. (chickpea) during early stages of water deficit: single or multi-factor controls. Plant J. 79:964-80. DOI: https://doi.org/10.1111/tpj.12599

Neugschwandtner R, Ziegler K, Kriegner S, Wagentristl H, Kaul HP, 2015. Nitrogen yield and nitrogen fixation of winter faba beans. Acta Agr. Scand. B-S. P. 65:658-66. DOI: https://doi.org/10.1080/09064710.2015.1042028

Palta JP, 1990. Stress interactions at the cellular and membrane levels. Hort. Sci. 25:1377. DOI: https://doi.org/10.21273/HORTSCI.25.11.1377

Razmjoo K, Heydarizadeh P, Sabzalian MR, 2008. Effect of salinity and drought stresses on growth parameters and essential oil content of Matricaria chamomile. Int. J. Agric. Biol. 10:451-4.

Šavicka M, Skute N, 2010. Effects of high temperature on malondialdehyde content, superoxide production and growth changes in wheat seedlings (Triticum aestivum L.). Ekologija. 56:26-33. DOI: https://doi.org/10.2478/v10055-010-0004-x

Shubhra JD, Goswami CL, Munjal R, 2004. Influence of phosphorus application on water relations, biochemical parameters and gum content in cluster bean under water deficit. Biol. Plant. 48:445-8. DOI: https://doi.org/10.1023/B:BIOP.0000041101.87065.c9

Siddique BMR, Hamid A, Islam MS, 2000. Drought stress effect on water relations of wheat. Bot. Bull. Acad. 41:35-9.

Silva EN, Ferreira-Silva SL, Viégas RA, Silveira JAG, 2010. The role of organic and inorganic solutes in the osmotic adjustment of drought-stressed jatropha curcas plants. Environ. Exp. Bot. 69:279-85. DOI: https://doi.org/10.1016/j.envexpbot.2010.05.001

Singh AK, Bharati RC, Manibhushan NC, Pedpati A, 2013. An assessment of faba bean (Vicia faba L.) current status and future prospect. African. J. Agric. Res. 8:6634-41.

Singh SK, Reddy VR, 2014. Combined effects of phosphorus nutrition and elevated carbon dioxide concentration on chlorophyll fluorescence, photosynthesis, and nutrient efficiency of cotton. J. Plant Nutr. Soil Sc. 177:892-902. DOI: https://doi.org/10.1002/jpln.201400117

Song FB, Dai JY, Gu WB, Li HY, 1995. Effect of water stress on leaf water status in maize. J. Jilin. Agric. Univ. 17:5-9. [in Chinese].

Sulieman S, Ha C Van, Schulze J, Tran, LSP, 2013. Growth and nodulation of symbiotic Medicago truncatula at different levels of phosphorus availability. J. Exp. Bot. 64:2701-12. DOI: https://doi.org/10.1093/jxb/ert122

Wang W, Vinocur B, Altman A, 2003. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta. 218:1-14. DOI: https://doi.org/10.1007/s00425-003-1105-5

Wang X, Yan X, Liao H, 2010. Genetic improvement for phosphorus efficiency in soybean: a radical approach. Ann. Bot. 106:215-22. DOI: https://doi.org/10.1093/aob/mcq029

Waraich EA, Ahmad R, Ashraf MY, 2011. Role of mineral nutrition in alleviation of drought stress in plants. Austral. J. Crop Sci. 5:764-77.

Zhu JK, 2002. Salt and drought stress signal transduction in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 53:247-73. DOI: https://doi.org/10.1146/annurev.arplant.53.091401.143329

Zlatev Z, Lidon FC, 2012. An overview on drought induced changes in plant growth, water relations and photosynthesis. Emir. J. Food Agr. 24:57. DOI: https://doi.org/10.9755/ejfa.v24i1.10599

Published
2020-10-13
Info
Issue
Section
Original Articles
Keywords:
Drought, glycine betaine, growth, legume, osmoregulation, phosphorus, water potential.
Statistics
  • Abstract views: 531

  • PDF: 185
  • HTML: 0
How to Cite
Oukaltouma , K., El Moukhtari , A., Lahrizi , Y., Mouradi , M., Farissi , M., Willems , A., Qaddoury, A., Bekkaoui , F., & Ghoulam, C. (2020). Phosphorus deficiency enhances water deficit impact on some morphological and physiological traits in four faba bean (<em>Vicia faba L.</em&gt;) varieties. Italian Journal of Agronomy, 16(1). https://doi.org/10.4081/ija.2020.1662