The response mechanism of the HVA1 gene in hulless barley under drought stress

  • Xiaohua Yao State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining; Qinghai Academy of Agricultural and Forestry Sciences, Xining; Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China.
  • Kunlun Wu State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining; Qinghai Academy of Agricultural and Forestry Sciences, Xining; Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China.
  • Youhua Yao State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining; Qinghai Academy of Agricultural and Forestry Sciences, Xining; Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China.
  • Jie Li State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining; Qinghai Academy of Agricultural and Forestry Sciences, China.
  • Youcheng Ren State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining; Qinghai Academy of Agricultural and Forestry Sciences, Xining; Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China.
  • Dezhao Chi | qhcdz@163.com State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining; Qinghai Academy of Agricultural and Forestry Sciences, Xining; Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining, China.

Abstract

HVA1, a member of LEA3 (late embryogenesis abundant protein, group 3), is closely related to water stress. However, the response of HVA1 to drought remains unknown in hulless barley. In this study, cultivars with high (Handizi), intermediate (Kunlun 12), and low (Dama) drought tolerance were selected from 28 hulless barley cultivars from the Tibet-Qinghai plateau to explore the drought response mechanism of HVA1. Then, HVA1 was cloned and the expression of the three cultivars was studied using exposure to polyethylene glycol (PEG) 6000. HVA1s in the three hulless barleys were highly homologous at the nucleotide and amino acid levels with over 99% identity. Real-time quantitative polymerase chain reaction showed that the expression level of HVA1 induced by PEG 6000 had a single peak curve in the three cultivars, but higher HVA1 transcript accumulation was seen in Handizi than in Kunlun 12 and Dama under the same drought stress. This result was also proved in eight hulless barleys. The expression level was a better predictor of drought resistance than the genetic structure of HVA1.

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Published
2017-12-11
Section
Original Articles
Keywords:
Drought stress, Expression level, Hulless barley, HVA1
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How to Cite
Yao, X., Wu, K., Yao, Y., Li, J., Ren, Y., & Chi, D. (2017). The response mechanism of the HVA1 gene in hulless barley under drought stress. Italian Journal of Agronomy, 12(4). https://doi.org/10.4081/ija.2017.804